What is the serological identification of bacteria?
Serological tests can be used to detect viral & bacterial antigens and antibodies (IgG and IgM), to help diagnose diseases and check immune status. A range of techniques are utilized including ELISA, chemiluminescence, agglutination, direct and indirect immunofluorescence, and Western blotting.
What tests are used to identify bacteria?
Tests used to identify Gram Positive Bacteria
- Catalase Test.
- Mannitol Salt Agar (MSA)
- Blood Agar Plates (BAP) Streak-stab technique.
- Taxos P (optochin sensitivity testing)
- Taxos A (bacitracin sensitivity testing)
- CAMP Test.
- Bile Esculin Agar.
- Nitrate Broth.
What are two methods of identifying bacteria?
Among the techniques we use are:
- DNA sequencing – to identify bacteria, moulds and yeasts.
- Riboprinter analysis – for bacterial identification and characterisation.
- Repeat–based polymerase chain reaction – for assessing the similarity of microorganisms.
- Rapid pathogen confirmation by polymerase chain reaction.
What are the biochemical test for bacteria identification?
Biochemical tests are among the most important methods for microbial identification. Routine biochemical tests include tests for carbohydrate fermentation (Figure 2.18(A)), methyl red (Figure 2.18(B)), citric acid utilization (Figure 2.18(C)), and hydrogen sulfide production (Figure 2.18(D)).
What is serology testing?
Antibody serology tests check for the presence or level of specific antibodies in the blood. Antibodies are proteins that your immune system makes to fight foreign substances. These substances are often pathogens, (disease-causing germs) such as viruses and bacteria.
How are serology tests done?
A blood sample collected in a vial is sent to a lab where a test is run to look for the presence of antibodies in the blood serum. The presence of antibodies means that the body had an immune response to fight off a virus.
What are the conventional methods for bacterial identification?
Conventionally identification of bacteria is usually performed by three methods including culture-based counting for colony-forming units (CFU) [22],[25], spectrophotometer method of optical density (OD) measurement [23],[24], and flow cytometry (FCM) [26],[27].
How do you identify a bacteria?
Bacteria are identified routinely by morphological and biochemical tests, supplemented as needed by specialized tests such as serotyping and antibiotic inhibition patterns. Newer molecular techniques permit species to be identified by their genetic sequences, sometimes directly from the clinical specimen.
What is IMViC test?
The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group. The term “IMViC” is an acronym for each of these tests. “I” is for indole test; “M” is for methyl red test; “V” is for Voges-Proskauer test, and “C” is for citrate test.
What does a MacConkey agar test for?
MacConkey agar is a selective and differential culture medium for bacteria. It is designed to selectively isolate Gram-negative and enteric (normally found in the intestinal tract) bacteria and differentiate them based on lactose fermentation.
What are the serology tests in the laboratory?
Serology tests in the laboratory include: 1 markers of Hepatitis B infection or past infection 2 Human Immunodeficiency Virus (HIV) status 3 Hepatitis C virus 4 Rubella immune status.
What is the importance of bacterial identification in health care?
Identify the disease: It helps to know the type of infection and the disease caused by the individual. Ex. Tuberculosis can be identified by an acid-fast stain test for mycobacterium. So bacterial identification is important in health care. 2. Select a suitable drug: Not all drugs (antibiotics) are active against all the bacteria.
What are the types of biochemical tests used for bacterial identification?
Catalase test, coagulase test, Oxidase test, Sugar fermentation test, Indoletest, Citrate Test, Urease test are the Biochemical tests used for bacterialidentification
What level is the laboratory for viral serology?
Viral serology and the molecular laboratory are situated on Level 6, although the immunoanalysers used by viral serology are part of the Core Automated Laboratory on Level 4. This section analyses blood samples for the presence of viral antigens and antibodies (IgG and IgM) to help diagnose diseases…