Why is the secondary antibody anti-mouse?
Invitrogen anti-mouse secondary antibodies are affinity-purified polyclonal antibodies with well-characterized specificity for mouse immunoglobulin classes, subclasses, and fragments. They are useful in the detection, sorting, or purification of the specified target (primary antibody).
What is anti rabbit IgG antibody?
Goat anti rabbit IgG antibody recognizes the Fc region of rabbit immunoglobulin G, confirmed by ELISA and immunoelectrophoresis. The antibody has not been species cross adsorbed and may cross react with IgG from other species. It shows weak recognition of rabbit IgM in ELISA.
What secondary antibody should I use?
Therefore, the secondary antibody should be an anti-IgG antibody that recognizes both heavy and light chain of the primary antibody (anti-IgG H&L). Primary monoclonal antibodies are normally raised in mouse, rat and Armenian hamster but even rabbit and human derived are also used.
Why are secondary antibodies conjugated?
Conjugated secondary antibodies are used to indirectly detect the target antigen upon binding to the unconjugated primary antibody and are used in an indirect immunoassay.
What are secondary antibodies used for?
Secondary antibodies are used for the indirect detection of a target to which a specific primary antibody is first bound. The secondary antibody must have specificity both for the antibody species as well as the isotype of the primary antibody being used.
What is the purpose of a secondary antibody?
A secondary antibody aids in the detection, sorting or purification of target antigens by binding to the primary antibody, which directly binds to the target antigen.
Could goat anti rabbit secondary antibodies be used to detect two different types of rabbit antibodies?
Yes, and especially if you have the primary antibodies of different isotype (for example IgG1 and IgG2a) recognizing A and B, then you can detect these with secondary IgG-specific goat anti-mouse antibodies conjugated to different Alexas, or whatever you wish.
What are good reasons for using a secondary conjugated antibody over a primary conjugated antibody in an immunoassay?
Advantages of using secondary antibodies Secondary antibodies are used for the indirect detection of a target to which a specific primary antibody is first bound. The secondary antibody must have specificity both for the antibody species as well as the isotype of the primary antibody being used.
What is an anti-rabbit secondary antibody?
Our anti-rabbit secondary antibodies are affinity-purified polyclonal or monoclonal antibodies with well-characterized specificity for rabbit immunoglobulin classes and fragments. They are available in host species including goat, donkey, mouse, chicken, and sheep.
What are fluorescent secondary antibodies used for?
Fluorescent Secondary Antibodies. Fluorescent-dye conjugated secondary antibodies provide a tool for identifying proteins in many applications including fluorescent cell imaging, western blotting, immunohistochemistry and more. The advantages of using a fluorescently labeled secondary antibody include brighter signal, multiplexing capabilities,
What are affinipure antibodies?
Jackson ImmunoResearch AffiniPure™ Anti-Rabbit secondary antibodies are affinity purified polyclonal antibodies with specificity for rabbit immunoglobulins. We offer Anti-Rabbit secondary antibodies in a range of host species including Alpaca, Donkey, Goat, Rabbit, Rat and Sheep.
What are superclonal secondary antibodies?
Thermo Scientific Superclonal secondary antibodies represent a breakthrough in recombinant antibody technology designed to provide precise and accurate detection of mouse, rabbit and goat primary antibodies in a variety of applications.