What is a BD bactec Peds Plus F culture vials?

The new BD BACTEC™ PEDS Plus™/F plastic blood culture bottle is designed for customer convenience for the qualitative culture and recovery of aerobic microorganisms from pediatric and non-pediatric blood specimens less than 5 mL in volume.

What is bactec bottle?

The BD BACTEC bottle portfolio provides a full line of blood culture media developed specifically for the detection of aerobes, anaerobes, yeast, fungi and mycobacteria to help improve time to detect and organism recovery2 from both adult and pediatric patients.

Which blood culture bottle do you fill first?

The blue (aerobic) blood culture bottle should be filled first, then the purple (anaerobic) bottle as the butterfly tubing may contain air. Air entering the purple bottle will impede the growth of anaerobic organisms.

What is the liquid in blood culture bottles?

Blood culture bottles contain a growth medium, which encourages microorganisms to multiply, and an anticoagulant that prevents blood from clotting. Sodium polyanethol sulfonate (SPS) is the most commonly used anticoagulant because it does not interfere with the growth of most organisms.

How does the bactec work?

The BD BACTEC™ Automated Blood Culture System utilizes fluorescent technology in detecting the growth of or- ganisms in the blood culture bottles. When microorganisms are present in the cultured vials, they metabolize nutrients in the culture medium, releasing carbon dioxide into the medi- um.

What is Pedsplus?

Pedsplus is the only areas’ Pediatric Practice that offers both Primary Care & After-hours Urgent Care for children & youth, 0-21Yrs. The Primary Care & Urgent Care have separate waiting room & exam rooms for well & sick/urgent care visits.

What is a bactec blood culture?

The BD BACTEC™ Automated Blood Culture System utilizes fluorescent technology in detecting the growth of organisms in the blood culture bottles. The sample to be tested is inoculated into one or more vials which are inserted into the BD Bactec fluorescent series instrument for incubation and periodic reading.

What color are blood culture tubes?


Blood Culture Bottles are ALWAYS drawn prior to other labs to reduce contamination. .
Royal Blue No additive (serum); special glass and stopper material 7.0 mL
Red No additive 7.0 mL
Light Blue 3.2% Sodium Citrate 4.5 mL

Which antiseptic do you use for blood cultures?

Among the evaluated antiseptics, isopropyl alcohol may be the optimal antiseptic for use prior to obtaining blood for culture, given its convenience, low cost, and tolerability.

What should you do before injecting the blood into the culture bottle?


  1. Locate the vein to be used.
  2. Remove Frepp™ from package.
  3. Place sponge on selected venipuncture site and depress once or twice to saturate sponge.
  4. Use a back and forth friction scrub for at least 30 seconds.
  5. Allow area prepared to dry for approximately 30 seconds.
  6. Proceed with collection of blood.

How many mL are in a blood culture bottle?

7. Ten ml of blood is optimal in each blood culture bottle. Do not overfill the bottles as this can lead to false-positive results due to excessive WBC’s. If less than 10 ml is obtained, 5 ml is placed into the aerobic (blue) blood culture bottle and the rest is placed into the anaerobic (purple) bottle.

What is the purpose of blood culture bottles?

A blood culture is a laboratory test in which blood, taken from the patient, is inoculated into bottles containing culture media to determine whether infection-causing microorganisms (bacteria or fungi) are present in the patient’s bloodstream.

Which blood culture bottle should be drawn first?

Adults: suspected bacteremia – draw two sets of blood culture bottles (aerobic and anaerobic) from two separate venipuncture sites. Maximum of 4 cultures. The aerobic bottle should be inoculated first as there is about 0.5 cc of air in the line of the collection set and sometimes it is difficult to obtain 8-10 cc of blood per bottle (15-20 cc/set).

How much are blood culture bottles?

What is a Blood Culture? • Blood cultures are ordered as a set, which consists of 2 bottles (1 aerobic bottle & 1 anaerobic bottle). The Contaminated Blood Culture • If the skin is not adequately cleansed before drawing blood for culture, bacteria on the skin will be injected into the bottle, producing a false positive blood culture.

What do additives do blood culture bottles contain?

What is inside the blood culture bottle? Basic medium contains nutrient broth and an anticoagulant (Anticoagulant sodium polyanethol sulfonate (SPS) is suggested; ethylenediaminetetraacetic (EDTA) acid and sodium citrate inhibit organisms. Additives may be added to support the growth of bacteria with no cell wall.)

How to collect blood culture?

First shave the skin,cleanse the venipuncture site with isopropanol.

  • Then use tincture of iodine or povidone iodine to disinfect the site using progressively larger concentric circles.
  • The venipuncture site must not be palpated after preparation.
  • Blood is then drawn. Following venipuncture,alcohol is used to remove the iodine from the site.