What is the function of cytochrome c peroxidase?
CCP can be derived from aerobically grown yeast strains and can be isolated in both native and recombinant forms with high yield from Saccharomyces cerevisiae. The enzyme’s primary function is to eliminate toxic radical molecules produced by the cell which are harmful to biological systems.
How do you test for cytochrome c?
The techniques currently used by most laboratories to measure cytochrome c release include Western blot and fluorescence micros- copy, both presenting several difficulties. The Western blot technique requires cell fractionation and isolation of mito- chondria (1,9–11).
What makes cytochrome c positive?
The binding of cytochrome c is in part facilitated by a cluster of positively charged lysine residues on the surface of the cytochrome c (cytochrome c also contains a heme which carries the electron from cytochrome c-1 to cytochrome c oxidase).
Does cytochrome c have enzymatic activity?
The bacterial cytochrome c oxidases of known structure contain one additional small protein subunit, called ‘subunit IV’. It has no counterpart in the mitochondrial enzyme and its function is not known.
What is the purpose of an oxidase test?
The oxidase test identifies organisms that produce the enzyme cytochrome oxidase. Cytochrome oxidase participates in the electron transport chain by transferring electrons from a donor molecule to oxygen.
What is cytochrome c assay?
The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase.
How is cytochrome c released?
Cytochrome c is often released from mitochondria during the early stages of apoptosis, although the precise mechanisms regulating this event remain unclear. Mitochondria play a central role in the initiation of apoptosis.
What does cytochrome c interact with?
In healthy cells, cytochrome c (Cyt c) is located in the mitochondrial intermembrane/intercristae spaces, where it functions as an electron shuttle in the respiratory chain and interacts with cardiolipin (CL).
How does cytochrome c work?
Cytochrome c is primarily known for its function in the mitochondria as a key participant in the life-supporting function of ATP synthesis. However, when a cell receives an apoptotic stimulus, cytochrome c is released into the cytosol and triggers programmed cell death through apoptosis.
What role does cytochrome c oxidase play in aerobic respiration?
Cytochrome c oxidase (COX) or complex IV of the mitochondrial respiratory chain plays a fundamental role in energy production of aerobic cells. This multimeric enzyme of the inner mitochondrial membrane catalyzes the last step of respiration, the transfer of electrons from cytochrome c to molecular oxygen.