What is a pGEX plasmid?

Plasmid: pGEX-1 Expression vector permitting production of a fusion protein. (ATCC staff) The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin.

What is pGEX?

pGEX-2TK is designed to allow the detection of expressed proteins by directly labeling the tagged products in vitro. This vector contains the recognition sequence for the catalytic subunit of cAMP-dependent protein kinase obtained from heart muscle.

What is pGEX 2t?

Bacterial vector for expressing GST fusion proteins with a thrombin site.

What is thrombin cleavage site?

A thrombin cleavage site (e.g., Leu-Val-Pro-Arg-ll-Gly-Ser; where ll denotes the cleavage site) is widely incorporated within the linker region of fusion or affinity tagged recombinant proteins. The kit contains active thrombin enzyme sufficient to cleave up to 5 mg of the target protein.

Is pUC19 an expression vector?

pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.

Does pGEX have Lac O?

All Answers (3) pGEX vectors such as, pGEX-3X and pGEX-2T, contains the Ptac (trp/lac) hybrid promoter, which is IPTG responsive.

How many amino acids are in thrombin?

Thrombin is a α/β heterodimer composed of a 36 amino acid A chain and 259 amino acid B chain connected by a disufide bridge between Cys1 and Cys122, in addition to 3 other intrachain disulfide bonds. Its overall fold is similar to trypsin and chymotrypsin and it belongs to the peptidase S1 protease family.

Why is pUC19 a good cloning vector?

pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. NEB offers a selection of common cloning plasmids and DNAs for use as substrates.