What are inhibitors of helicase?
HCV helicase inhibitors The commercially available dye, soluble blue HT binds the NS3 ATP-binding cleft to inhibit the enzyme. Nucleoside and base analogs known as benzotriazoles inhibit HCV helicase in vitro ,with IC50s ranging from 2 to 20 µM, and cellular HCV RNA replication with IC50s of approximately 10–60 µM.
What is the role of the enzyme helicase?
DNA helicases catalyze the disruption of the hydrogen bonds that hold the two strands of double-stranded DNA together. This energy-requiring unwinding reaction results in the formation of the single-stranded DNA required as a template or reaction intermediate in DNA replication, repair and recombination.
What does helicase do in bacteria?
DnaB helicase is an enzyme in bacteria which opens the replication fork during DNA replication.
Is helicase a restriction enzyme?
Helicases were defined classically as ATPases that use directional translocation to unwind duplex nucleic acids. Pseudo-helicases include the ATP-dependent bacterial Type I and III restriction enzymes (REs) which use ATP hydrolysis to communicate between two distant restriction sites on the same DNA (5,6).
What happens when DNA helicase is inhibited?
(A) Small molecule helicase inhibitors may interfere with the catalytic activities of DNA helicase proteins and their molecular and cellular functions by a variety of mechanisms. A helicase-interacting compound may disrupt protein oligomerization, binding to DNA substrate, or compete with ATP binding.
What is the main function of ligase?
Ligases are enzymes that are capable of catalyzing the reaction of joining two large molecules by establishing a new chemical bond, generally with concomitant hydrolysis of a small chemical group on one of the bulky molecules or simply linking of two compounds together (e.g., enzymes that catalyze joining of C–O, C–S.
What enzyme removes primers?
DNA polymerase I
Removal of RNA primers and joining of Okazaki fragments. Because of its 5′ to 3′ exonuclease activity, DNA polymerase I removes RNA primers and fills the gaps between Okazaki fragments with DNA.
What does DNA helicase do during transcription?
Helicase is an enzyme that unwinds DNA strands. After it unwinds double-stranded DNA, two pairs of single-stranded DNA are available for replication, transcription and translation. Helicase is also known as a motor protein due to its ability to move down the double-stranded DNA strand and unzip it into two single strands.
What does acetylcholinesterase inhibitor mean?
Acetylcholinesterase inhibitor. An acetylcholinesterase inhibitor or anti-cholinesterase is a chemical that inhibits the acetylcholinesterase enzyme from breaking down acetylcholine, thereby increasing both the level and duration of action of the neurotransmitter acetylcholine. Reversible, quasi-irreversible and irreversible inhibitors exist.
What is a histone deacetylase inhibitor?
Histone deacetylase inhibitors (HDAC inhibitors) are a group of chemotherapy agents used to treat hematological cancers. The US Food and Drug Administration has approved the use of four different HDAC inhibitors to treat T-cell lymphoma and myeloma.
What is a RNA inhibitor?
RNA inhibitors. RNA inhibitors such as rifampin , act upon DNA-dependent RNA polymerase. Antifolates (DNA, RNA, and protein) Antifolates act primarily as inhibitors of both RNA and DNA, and are often grouped with nucleic acid inhibitors in textbooks.